Active Recombinant Mouse NTRK1 protein(Met1-Gly420), His-tagged

• Cat.No.: NTRK1-415M
• Product Overview: Recombinant Mouse NTRK1 (NP_001028296.1)(Met1-Gly420) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Gly420
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 10 ng/mL of recombinant mouse NGF.
• Molecular Mass: The recombinant mouse NTRK1 comprises 398 amino acids and has a predicted molecular mass of 43.7 kDa. The apparent molecular mass of the protein is approximately 69 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ntrk1 neurotrophic tyrosine kinase, receptor, type 1 [ Mus musculus ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; Tkr; trk; TrkA; C80751
• Gene ID: 18211
• mRNA Refseq: NM_001033124
• Protein Refseq: NP_001028296