Recombinant Mouse Ctf1 protein

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Recombinant Mouse Ctf1 protein

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Cat.No. : Ctf1-658M
Product Overview : Recombinant Mouse Ctf1 protein was expressed in Escherichia coli.
Description : Cardiotrophin­1 (CT­1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Murine CT­1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and murine CT­1 share 80 % a.a. sequence identity and exhibit cross-species activity.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Molecular Mass : Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.
Protein length : 203
AA Sequence : MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA
Endotoxin : Less than 1 EU/µg of rMuCT-1 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of Ctf1-658M :

Gene Name : Ctf1
Official Symbol : Ctf1
Synonyms : CTF1; cardiotrophin 1; cardiotrophin-1; CT-1;
Gene ID : 13019
mRNA Refseq : NM_007795
Protein Refseq : NP_031821
UniProt ID : Q60753

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