| Cat. No.: | PDGFA-56H |
| Product Overview: | Recombinant Human PDGF-AA produced inYeastis a homodimeric, glycosilated, polypeptide chain containing 2 x 110 amino acids and having a total molecular mass of 34 kDa. PDGF-AA is purified by proprietary chromatographic techniques. |
| Description: | PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. |
| Source: | Yeast. |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity: | Greater than 98.0% as determined by SDS-PAGE. |
| Formulation: | The protein was lyophilized with 20mM sodium phosphate buffer. |
| Solubility: | It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Biological Activity: | The ED50was found to be 1ng/ml calculated by the ability to stimulate the proliferation of mouse 3T3 fibroblasts. (PNAS 94, 10205, 1997. Biochemistry, 1996, 35, 12077). |
| Protein Content: | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard. |
| Storage: | Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Gene Name: | PDGFA platelet-derived growth factor alpha polypeptide [ Homo sapiens ] |
| Synonyms: | PDGFA; platelet-derived growth factor alpha polypeptide, 2 x 110 amino acids; PDGF1; PDGF-A; Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor A chain; PDGF A-chain; PDGF subunit A; platelet-derived growth factor alpha; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform; preproprotein |
| Gene ID: | 5154 |
| mRNA Refseq: | NM_002607 |
| Protein Refseq: | NP_002598 |
| UniProt ID: | P04085 |
| Chromosome Location: | 7p22 |
| MIM: | 173430 |
| Pathway: | Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by PDGF |
| Function: | cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; contributes_to platelet-derived growth factor receptor binding; protein heterodimerization activity |
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