Active Recombinant Rat Pdgfa protein

Species :	Rat
Source :	E.coli
Tag :	Non
Protein Length :	87-196 aa
Form :	Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10^5 IU/mg.
Molecular Mass :	Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).
AASequence :	MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
Endotoxin :	Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.
Purity :	>98% by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	Pdgfa platelet-derived growth factor alpha polypeptide [ Rattus norvegicus ]
Official Symbol	Pdgfa
Synonyms	PDGFA; platelet-derived growth factor alpha polypeptide; platelet-derived growth factor subunit A; PDGF-1; PDGF subunit A; platelet derived growth factor, alpha; Platelet-derived growth factor A chain; PDGFACP;
Gene ID	25266
mRNA Refseq	NM_012801
Protein Refseq	NP_036933