Recombinant Human B2M, C13&N15-labeled

• Cat.No.: B2M-218H
• Product Overview: Recombinant Human B2M MS Standard Protein, C13 and N15-labeled (B2M, Heavy Labeled) Ile 21 - Met 119 was produced in human 293 cells (HEK293).

Specification

• Description: β2 microglobulin, also known as Beta-2-microglobulin (B2M), is a component of MHC class I molecules which belongs to the beta-2-microglobulin family. B2M is present on all nucleated cells (excludes red blood cells). B2M associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. An additional function of B2M is association with the HFE protein, together regulating the expression of hepcidin in the liver which targets the iron transporter ferroportin on the cytoplasmic membrane of enterocytes and macrophages for degradation resulting in decreased iron uptake from food and iron release from recycled red blood cells respectively. Loss of this function causes iron excess and hemochromatosis. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP).
• Source: HEK293
• Species: Human
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: B2M, Heavy Labeled, fused with 6×His tag at the C-terminus, has a calculated MW of 12.6 kDa. The predicted N-terminus is Ile 21. DTT-reduced Protein migrates as 13 kDa.
• Endotoxin: Less than 1.0 EU per μg of the B2M, Heavy Labeled by the LAL method.
• Purity: >98% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).

Gene Information

• Gene Name: B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol: B2M
• Synonyms: B2M; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• Gene ID: 567
• mRNA Refseq: NM_004048
• Protein Refseq: NP_004039
• MIM: 109700
• UniProt ID: P61769
• Chromosome Location: 15q21-q22.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Amyloids, organism-specific biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and
• Function: protein binding