- Bovine(1)
- Chicken(2)
- Human(13)
- Macaca mulatta(1)
- Mouse(5)
- Rabbit(1)
- Rat(4)
- Rhesus Macaque(2)
Recombinant Mouse CCL20 cell lysate
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Cat.No. : | CCL20-447MCL |
Product Overview : | Mouse MIP3A / CCL20 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
Source : | Baculovirus-Insect Cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Ccl20 chemokine (C-C motif) ligand 20 [ Mus musculus ] |
Official Symbol : | CCL20 |
Synonyms : | CCL20; chemokine (C-C motif) ligand 20; C-C motif chemokine 20; MIP-3-alpha; CC chemokine LARC; CC chemokine ST38; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; small inducible cytokine subfamily A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1; |
Gene ID : | 20297 |
mRNA Refseq : | NM_001159738 |
Protein Refseq : | NP_001153210 |
Pathway : | Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem; |
Function : | chemokine activity; cytokine activity; protein binding; |
◆ Recombinant Protein
CCL20-793M | Recombinant Mouse CCL20 Protein (Ala28-Met97) | +Inquiry |
CCL20-22H | Recombinant Human CCL20 Protein | +Inquiry |
CCL20-0624H | Recombinant Human CCL20 Protein, GST-Tagged | +Inquiry |
CCL20-517H | Recombinant Human CCL20 Protein, HIgG1 Fc-tagged | +Inquiry |
Ccl20-2032M | Recombinant Mouse Ccl20 Protein | +Inquiry |
|
CCL1 CCL11 Ccl12 CCL13 CCL14 CCL15 CCL16 CCL17 CCL18 CCL19 CCL2 CCL20 CCL21 Ccl21c CCL22 CCL23 CCL24 CCL25 CCL26 CCL27 Ccl27a CCL28 CCL3 CCL3L1 Ccl4 CCL5 Ccl6 CCL7 CCL8 Ccl9 CCL4L2 Ccl21a CCL4L1 CCL3L3 Ccl21b Ccl27b
Easy access to products and services you need from our library via powerful searching tools.
Copyright © 2022 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy