Recombinant Mouse CCL20 cell lysate

• Cat.No.: CCL20-447MCL
• Product Overview: Mouse MIP3A / CCL20 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Ccl20 chemokine (C-C motif) ligand 20 [ Mus musculus ]
• Official Symbol: CCL20
• Synonyms: CCL20; chemokine (C-C motif) ligand 20; C-C motif chemokine 20; MIP-3-alpha; CC chemokine LARC; CC chemokine ST38; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; small inducible cytokine subfamily A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
• Gene ID: 20297
• mRNA Refseq: NM_001159738
• Protein Refseq: NP_001153210
• Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem
• Function: chemokine activity; cytokine activity; protein binding

Reviews

08/25/2020

Expert protein engineering support, essential for our projects' progress.

05/14/2020

Consistent western blot results, an indispensable tool in our lab.

08/22/2019

Dependable protein quantification, ensures data precision in our work.

Q&As

What are the mechanisms by which CCL20 modulates the migration and differentiation of dendritic cells?

CCL20 influences dendritic cell migration and differentiation, crucial for initiating immune responses.

How does CCL20 contribute to tumor progression and the formation of the tumor microenvironment in cancer?

CCL20 contributes to cancer progression by shaping the tumor microenvironment and aiding tumor cell invasion.

What role does CCL20 play in the pathogenesis of autoimmune diseases like rheumatoid arthritis?

CCL20 plays a significant role in autoimmune diseases by attracting inflammatory cells to affected joints.

How does CCL20 influence wound healing and tissue repair processes?

CCL20 is involved in wound healing and tissue repair, facilitating immune cell migration and tissue regeneration.

How do alterations in CCL20 expression or signaling affect skin homeostasis and the development of skin diseases?

Changes in CCL20 expression impact skin health, potentially contributing to the development of skin disorders.

How does CCL20 mediate the recruitment and activation of immune cells, particularly in inflammatory conditions?

CCL20 recruits and activates immune cells to sites of inflammation, coordinating the immune response.

What is the impact of CCL20 on mucosal immunity, especially in the gut and respiratory tract?

CCL20 impacts mucosal immunity, playing a critical role in immune defense in the gut and respiratory system.