Recombinant Human MTHFD2, MYC/DDK-tagged

• Cat.No.: MTHFD2-394H
• Product Overview: Recombinant Human MTHFD2, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
• Source: HEK293 cells
• Species: Human
• Tag: MYC/DDK
• Molecular Mass: 34.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase [ Homo sapiens ]
• Official Symbol: MTHFD2
• Synonyms: MTHFD2; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase; NMDMC; methylenetetrahydrofolate dehydrogenase 2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; EC 1.5.1.15
• Gene ID: 10797
• mRNA Refseq: NM_006636
• Protein Refseq: NP_006627
• MIM: 604887
• UniProt ID: P13995
• Chromosome Location: 2p13.1
• Pathway: C1-unit interconversion; Nucleotide Metabolism; One carbon pool by folate; tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
• Function: NOT formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity