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Recombinant Human PLK4 293 Cell Lysate

Cat.No. : PLK4-3102HCL
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  • Gene Information
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Description : Antigen standard for polo-like kinase 4 (Drosophila) (PLK4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PLK4 polo-like kinase 4 [ Homo sapiens ]
Official Symbol : PLK4
Synonyms : PLK4; polo-like kinase 4; polo like kinase 4 (Drosophila) , serine/threonine kinase 18 , STK18; serine/threonine-protein kinase PLK4; Sak; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak; SAK; STK18;
Gene ID : 10733
mRNA Refseq : NM_001190799
Protein Refseq : NP_001177728
MIM : 605031
UniProt ID : O00444
Chromosome Location : 4q27-q28
Pathway : Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem; Mitotic G2-G2/M phases, organism-specific biosystem;
Function : ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Is the interaction between PLK4 protein and other proteins? 05/21/2023

There is an interaction between PLK4 protein and other proteins. For example, it interacts with certain proteins on spindle fibers to regulate chromosome segregation, and with other proteins in signal transduction pathways to regulate cell proliferation and differentiation.

What is the function of PLK4 protein in cell division? 12/04/2020

PLK4 protein is mainly responsible for regulating the process of chromosome segregation and cytoplasmic division in cell division. It is associated with the assembly and function of spindle fibers, affecting the accuracy of chromosome segregation and cell division.

Which biological molecules can be used as molecular markers of PLK4 protein? 11/30/2020

Certain biomolecules can be used as molecular markers of PLK4 protein, such as mRNA or protein of PLK4 protein detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

What is the current stage of research for the treatment of PLK4 protein? 08/11/2020

Therapeutics targeting the PLK4 protein are still in the early stages of research. A number of small molecule inhibitors and antibody drugs have been developed and initially evaluated in laboratories and clinical trials. However, further research and clinical validation of these treatments are needed to determine their safety and efficacy.

What are the therapeutic strategies for PLK4 protein? 08/04/2020

Therapeutic strategies for PLK4 protein include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against the PLK4 protein to inhibit its aberrant activity in tumors, or the regulation of PLK4 protein expression through gene knockout or overexpression techniques.

Does abnormal PLK4 protein expression cause disease? 06/07/2020

Studies have shown that the abnormal expression of PLK4 protein is associated with the occurrence of a variety of tumors, including but not limited to brain cancer, lung cancer, breast cancer, pancreatic cancer, etc. It may be related to aspects such as proliferation, metastasis, and drug resistance of tumor cells.

Customer Reviews (3)

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Reviews
06/17/2023

    With PLK4, the sensitivity of the experiment was significantly improved.

    04/08/2022

      Strong anti-pollution ability, not easy to pollute, to ensure the accuracy of the experiment.

      11/07/2020

        The method is very simple, and it is easy to obtain accurate experimental results.

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