Recombinant Human PLK4 293 Cell Lysate

• Cat.No.: PLK4-3102HCL

Specification

• Description: Antigen standard for polo-like kinase 4 (Drosophila) (PLK4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PLK4 polo-like kinase 4 [ Homo sapiens ]
• Official Symbol: PLK4
• Synonyms: PLK4; polo-like kinase 4; polo like kinase 4 (Drosophila) , serine/threonine kinase 18 , STK18; serine/threonine-protein kinase PLK4; Sak; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak; SAK; STK18
• Gene ID: 10733
• mRNA Refseq: NM_001190799
• Protein Refseq: NP_001177728
• MIM: 605031
• UniProt ID: O00444
• Chromosome Location: 4q27-q28
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem; Mitotic G2-G2/M phases, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity