"HDAC2" Related Products

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Recombinant Human HDAC2 293 Cell Lysate

Cat.No.: HDAC2-5605HCL
Description: Antigen standard for histone deacetylase 2 (HDAC2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HDAC2 histone deacetylase 2 [ Homo sapiens ]
Official Symbol: HDAC2
Synonyms: HDAC2; histone deacetylase 2; RPD3; YAF1; YY1-associated factor 1; transcriptional regulator homolog RPD3; HD2;
Gene ID: 3066
mRNA Refseq: NM_001527
Protein Refseq: NP_001518
MIM: 605164
UniProt ID: Q92769
Chromosome Location: 6q21
Pathway: Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Chronic myeloid leukemia, organism-specific biosystem; Chronic myeloid leukemia, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem;
Function: NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; enzyme binding; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); hydrolase activity; protein binding; protein deacetylase activity; sequence-specific DNA binding; transcription factor binding;

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