Recombinant Human IGF1R protein, His-tagged
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|Product Overview :||Recombinant Human IGF1R (Glu31-Asn932) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.|
|Predicted N Terminal :||Glu31|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 104.8 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Glu31-Asn932|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of IGF1R-180H :||
Human IGF-I R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of IGF1R-180H :||
Immobilized Human IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.04-0.313 μg/mL (QC tested).
Immobilized Human IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-II, Avitag,Fc Tag with a linear range of 0.156-5 μg/mL (Routinely tested).
|Gene Name :||IGF1R|
|Official Symbol :||IGF1R|
|Synonyms :||IGF1R; insulin-like growth factor 1 receptor; CD221; IGFIR; IGFR; JTK13; MGC18216; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor; MGC142170; MGC142172|
|Gene ID :||3480|
|mRNA Refseq :||NM_000875|
|Protein Refseq :||NP_000866|
|UniProt ID :||P08069|
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|IGF1R-1392H||Recombinant Human IGF1R Protein, MYC/DDK-tagged||+Inquiry|
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|IGF1R-2928HCL||Recombinant Human IGF1R cell lysate||+Inquiry|
◆ Assay kits
|Kit-1600||IGF1R Functional Assay Kit||+Inquiry|
|Kit-1601||HEK 293 IGF1R Bioassay Kit||+Inquiry|