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| Cat.No.: | FN1-3B |
| Product Overview: | Purified Natural Bovine Fibronectin is purified from bovine plasma by affinity chromatography on gelatin-Sepharose. |
| Description: | Fibronectin is a high-molecular weight (~440kDa) extracellular matrix glycoprotein that binds to membrane-spanning receptor proteins called integrins.[1] In addition to integrins, fibronectin also binds extracellular matrix components such as collagen, fibrin and heparan sulfate proteoglycans (e.g. syndecans). Fibronectin exists as a dimer, consisting of two nearly identical monomers linked by a pair of disulfide bonds. The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms. |
| Source: | bovine plasma |
| Species: | Bovine |
| Form: | Supplied as a 0.2 μm filtered solution in 0.4 M Urea, 50 mM Tris-HCl (pH 7.5) and 0.5 M NaCl, at a concentration of 1.36 mg/mL. |
| Bio-activity: | Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1 - 5 μg/cm2. Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5 - 50 μg/mL. |
| Endotoxin: | <1.0 eu per 1 μg of the protein by the lal</1.0 |
| Purity: | >90%, as determined by SDS-PAGE and visualized by silver stain. |
| Applications: | Adherent Cell Assays |
| Usage: | FOR RESEARCH USE ONLY |
| Quality Control Test: | Samples are stable for up to six months at 2 - 8 centigrade. Avoid vortexing and excessive agitation. Aliquot into 50 μL to 100 μL volumes and store at ≤ -20 centigrade for up to six months in a manual defrost freezer. |
| Concentration: | 1.36 mg/mL |
| Warning: | Avoid repeated freeze-thaw cycles. |
| Gene Name: | FN1 fibronectin 1 [ Bos taurus ] |
| Official Symbol: | FN1 |
| Synonyms: | FN; fibronectin; fibronectin ED-A- region; fibronectin variable region; embryo-specific fibronectin 1 transcript; cumulus cell-specific fibronectin 1 transcript |
| Gene ID: | 280794 |
| mRNA Refseq: | NM_001163778 |
| Protein Refseq: | NP_001157250 |
| Chromosome Location: | chromosome: 2 |
| Pathway: | Amoebiasis, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem |
| Function: | heparin binding; peptidase activator activity |
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