"POLK" Related Products

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Recombinant Human DNA polymerase kappa

Cat.No.: POLK-137H
Product Overview: This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa), and highly purified by several steps of chromatography. The product is catalytically active and its molecular weight is 65 kD. Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
Description: Mammalian DNA polymerase κ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10^-3 and 2 x 10^-3, respectively. These error rates are high when compared to those of most other DNA polymerases. DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions.
Source: E. coli
Species: Human
Tag: His
Form: 0.2 M NaCl, 10 mM sodium phosphate buffer (pH 7.0), 50% glycerol
Purity: Over 90% by SDS-PAGE (CBB staining)
Applications: 1) Functional studies on translesion DNA synthesis by DNA polymerase kappa 2) Antigen for western blotting and ELISA by anti-Pol kappa antibody
Storage: Sent at 4℃ or at -20℃. Upon arrival, centrifuge briefly and store at -20℃ or at -80℃ for longer storage.
Concentration: 3.2 mg/ml as measured by BCA method
Gene Name: POLK polymerase (DNA directed) kappa [ Homo sapiens ]
Official Symbol: POLK
Synonyms: POLK; polymerase (DNA directed) kappa; DINB1; DNA polymerase kappa; DINB protein; DINP; POLQ;
Gene ID: 51426
mRNA Refseq: NM_016218
Protein Refseq: NP_057302
MIM: 605650
UniProt ID: Q9UBT6
Chromosome Location: 5q13
Pathway: Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem;
Function: DNA-directed DNA polymerase activity; damaged DNA binding; metal ion binding; nucleotidyltransferase activity; transferase activity;

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