Recombinant Human DNA polymerase kappa POLK-137H

Recombinant Human DNA polymerase kappa


Home / Products / Recombinant Proteins / Recombinant Human DNA polymerase kappa

Recombinant Human DNA polymerase kappa

POLK Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : POLK-137H
Product Overview : This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa), and highly purified by several steps of chromatography. The product is catalytically active and its molecular weight is 65 kD. Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
Description : Mammalian DNA polymerase κ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10^-3 and 2 x 10^-3, respectively. These error rates are high when compared to those of most other DNA polymerases. DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions.
Source : E. coli
Species : Human
Tag : His
Form : 0.2 M NaCl, 10 mM sodium phosphate buffer (pH 7.0), 50% glycerol
Purity : Over 90% by SDS-PAGE (CBB staining)
Applications : 1) Functional studies on translesion DNA synthesis by DNA polymerase kappa 2) Antigen for western blotting and ELISA by anti-Pol kappa antibody
Storage : Sent at 4℃ or at -20℃. Upon arrival, centrifuge briefly and store at -20℃ or at -80℃ for longer storage.
Concentration : 3.2 mg/ml as measured by BCA method
Gene Name : POLK polymerase (DNA directed) kappa [ Homo sapiens ]
Official Symbol : POLK
Synonyms : POLK; polymerase (DNA directed) kappa; DINB1; DNA polymerase kappa; DINB protein; DINP; POLQ;
Gene ID : 51426
mRNA Refseq : NM_016218
Protein Refseq : NP_057302
MIM : 605650
UniProt ID : Q9UBT6
Chromosome Location : 5q13
Pathway : Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem;
Function : DNA-directed DNA polymerase activity; damaged DNA binding; metal ion binding; nucleotidyltransferase activity; transferase activity;

Online Inquiry

  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy