Recombinant Human DNA polymerase kappa
Cat.No. : | POLK-137H |
Product Overview : | This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa), and highly purified by several steps of chromatography. The product is catalytically active and its molecular weight is 65 kD. Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold. |
- Specification
- Gene Information
- Related Products
Description : | Mammalian DNA polymerase κ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10^-3 and 2 x 10^-3, respectively. These error rates are high when compared to those of most other DNA polymerases. DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | 0.2 M NaCl, 10 mM sodium phosphate buffer (pH 7.0), 50% glycerol |
Purity : | Over 90% by SDS-PAGE (CBB staining) |
Applications : | 1) Functional studies on translesion DNA synthesis by DNA polymerase kappa 2) Antigen for western blotting and ELISA by anti-Pol kappa antibody |
Storage : | Sent at 4℃ or at -20℃. Upon arrival, centrifuge briefly and store at -20℃ or at -80℃ for longer storage. |
Concentration : | 3.2 mg/ml as measured by BCA method |
Gene Name : | POLK polymerase (DNA directed) kappa [ Homo sapiens ] |
Official Symbol : | POLK |
Synonyms : | POLK; polymerase (DNA directed) kappa; DINB1; DNA polymerase kappa; DINB protein; DINP; POLQ; |
Gene ID : | 51426 |
mRNA Refseq : | NM_016218 |
Protein Refseq : | NP_057302 |
MIM : | 605650 |
UniProt ID : | Q9UBT6 |
Chromosome Location : | 5q13 |
Pathway : | Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; |
Function : | DNA-directed DNA polymerase activity; damaged DNA binding; metal ion binding; nucleotidyltransferase activity; transferase activity; |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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