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Recombinant Human GDF11

Cat.No.: GDF11-1078H
Product Overview: Recombinant Human GDF11 (Accession # O95390) Asn299-Ser407, fused with an N-terminal Met, was produced in E. coli.
Source: E. coli
Species: Human
Predicted N Terminal: Met-Asn299-Leu-Gly-Leu-Asp-(Cys)-Asp-Glu-His
Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50
Molecular Mass: Recombinant Human GDF11, Met-tagged has a calculated MW of 12.6 kDa. In SDS-PAGE migrates as 13 kDa, reducing conditions.
Protein length: Asn299-Ser407
Purity: Assessed by mass spectrometry, and is >95% pure by SDS-PAGE under reducing conditions visualized by silver stain (see below).
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Gene Name: GDF11 growth differentiation factor 11 [ Homo sapiens ]
Official Symbol: GDF11
Synonyms: GDF11; growth differentiation factor 11; growth/differentiation factor 11; BMP 11; GDF-11; bone morphogenetic protein 11; BMP11; BMP-11;
Gene ID: 10220
mRNA Refseq: NM_005811
Protein Refseq: NP_005802
MIM: 603936
UniProt ID: O95390
Chromosome Location: 12q13.13

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