|Cat. No. :
|Product Overview :
||Recombinant Human ATG5 autophagy related 5 homolog produced inE.Coliis a non-glycosylated, polypeptide chain having a molecular weight of 31.6 kDa. The rhAPG5 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
||Mammalian cells undergoing apoptosis express high levels of APG5L (or ASP) protein relatively late in the apoptotic process. APG5L protein contains a high number of acidic residues, a potential glycosylation site, and potential casein kinase II, protein kinase C, and tyrosine kinase sites.
|Physical Appearance :
||Sterile Filtered clear solution.
||Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.
||Recombinant Human APG5 at a concentration of 0.1mg/ml containing 10 mM Tris, pH 8.0, 0.01% Triton X-100 and 0.002% NaN3 and 10mM DTT.
||• ELISA • MS• Western Blotting.
||RhAPG5 although stable at 14°Cfor 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.