||Recombinant Human Interleukin 31 expressed inE. Coliis a 15.8 kDa protein containing 141 amino acid residues.
||Human IL-31 is a T-cell derived cytokine that shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. It signals through a receptor complex comprised of GPL (GP130-like, IL-31RA) and OSMR (Oncostatin M receptor). GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases.
|Amino Acid Sequence:
||SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
||>98% by SDS-Page analysis and HPLC analysis. Endotoxin level less than 0.1ng/ug (1EU/ug).
||We recommend a quick spin followed by reconstitution in sterile DI water to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium containing serum and stored at 4°C(up to 1 week) or -20°Clong term).
||The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted IL-31 should be stored in working aliquots at -20°C. Avoid frequent freeze thaw cycles.