Recombinant Cynomolgus IL31 protein, His-tagged

• Cat.No.: IL31-312C
• Product Overview: Recombinant Cynomolgus IL31 protein(G7PJ78-1)(Leu27-Thr163), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Leu27-Thr163
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus IL-31, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.59 ug/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 16.5 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.