| Species : |
Human |
| Source : |
Sf9 Cells |
| Tag : |
Non |
| Description : |
The human BRCA1, the product of breast/ovary cancer gene 1, is a hyperphosphorylated protein functioning as a tumor suppressor involved in both transcription regulation and DNA repair. BRCA1 associates with RAD51 and has shown to be required for transcription- coupled repair of DNA damage as well as for the repair of chromosomal double-strand breaks. Association with a human SWI/SNF-related complex has recently suggested a potential role of BRCA1 in linking chromatin remodeling to breast cancer. Dysfunction of BRCA sensitizes cells to the inhibition of PARP [Poly(ADP-ribose) polymerase] enzymatic activity, resulting in chromosomal instability, cell cycle arrest, and subsequent apoptosis. The wild type BRCA1 (1-1863 residues) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity. |
| Form : |
Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol. |
| Purity : |
> 95% by SDS-PAGE. |
| Activity : |
1 ng is sufficient for a gel mobility shift assay in a 20 µl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay. |
| Application : |
Recombinant BRCA1 can be used 1) for in vitro function studies including transcription and DNA repair 2) for protein-protein interaction assay 3) for cell growth assay. |
| Usage : |
For in vitro use only. |
| Storage : |
Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles |
