||Recombinant full length human PARP , expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow PARP automodification by poly(ADP-ribose).
||This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
||Lyophilised:To reconstitute,add 100μl distilled water and leave for 5 min. Vortex gently for 1 min, avoiding air bubbles. Spin briefly.
||Preservative: NoneConstituents (upon reconstitution in 100ul distilled water): 50mM Tris, 100mM Sodium chloride, 10mM Magnesium chloride, 1mM DTT, 1mM NAD , 10 μg/mL activated DNA, plus lyophilization stabilizers. pH 8.
||Store at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.
||Contains 1 BRCT domain.Contains 1 PARP alpha-helical domain.Contains 1 PARP catalytic domain.Contains 2 PARP-type zinc fingers.