Recombinant Human PARP1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: PARP1-075H
• Product Overview: PARP1 MS Standard C13 and N15-labeled recombinant protein (NP_001609) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 112.9 kDa
• AA Sequence: MAESSDKLYRVEYAKSGRASCKKCS ESIPKDSLRMAIMVQSPMFDGKVPH WYHFSCFWKVGHSIRHPDVEVDGFS ELRWDDQQKVKKTAEAGGVTGKGQD GIGSKAEKTLGDFAAEYAKSNRSTC KGCMEKIEKGQVRLSKKMVDPEKPQ LGMIDRWYHPGCFVKNREELGFRPE YSASQLKGFSLLATEDKEALKKQLP GVKSEGKRKGDEVDGVDEVAKKKSK KEKDKDSKLEKALKAQNDLIWNIKD ELKKVCSTNDLKELLIFNKQQVPSG ESAILDRVADGMVFGALLPCEECSG QLVFKSDAYYCTGDVTAWTKCMVKT QTPNRKEWVTPKEFREISYLKKLKV KKQDRIFPPETSASVAATPPPSTAS APAAVNSSASADKPLSNMKILTLGK LSRNKDEVKAMIEKLGGKLTGTANK ASLCISTKKEVEKMNKKMEEVKEAN IRVVSEDFLQDVSASTKSLQELFLA HILSPWGAEVKAEPVEVVAPRGKSG AALSKKSKGQVKEEGINKSEKRMKL TLKGGAAVDPDSGLEHSAHVLEKGG KVFSATLGLVDIVKGTNSYYKLQLL EDDKENRYWIFRSWGRVGTVIGSNK LEQMPSKEDAIEHFMKLYEEKTGNA WHSKNFTKYPKKFYPLEIDYGQDEE AVKKLTVNPGTKSKLPKPVQDLIKM IFDVESMKKAMVEYEIDLQKMPLGK LSKRQIQAAYSILSEVQQAVSQGSS DSQILDLSNRFYTLIPHDFGMKKPP LLNNADSVQAKAEMLDNLLDIEVAY SLLRGGSDDSSKDPIDVNYEKLKTD IKVVDRDSEEAEIIRKYVKNTHATT HNAYDLEVIDIFKIEREGECQRYKP FKQLHNRRLLWHGSRTTNFAGILSQ GLRIAPPEAPVTGYMFGKGIYFADM VSKSANYCHTSQGDPIGLILLGEVA LGNMYELKHASHISKLPKGKHSVKG LGKTTPDPSANISLDGVDVPLGTGI SSGVNDTSLLYNEYIVYDIAQVNLK YLLKLKFNFKTSLWTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PARP1 poly(ADP-ribose) polymerase 1 [ Homo sapiens (human) ]
• Official Symbol: PARP1
• Synonyms: PARP1; poly(ADP-ribose) polymerase 1; ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; protein poly-ADP-ribosyltransferase PARP1; EC 2.4.2.30
• Gene ID: 142
• mRNA Refseq: NM_001618
• Protein Refseq: NP_001609
• MIM: 173870
• UniProt ID: P09874