||Recombinant full-length human CSK was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW= 78 kDa.
||CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src through tyrosine phosphorylation of the c-src carboxy terminus. A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells.
||Sf9 insect cellsusing baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.