| Description : |
The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Bio-activity : |
CSK activity verified in a biochemical assay: CSK (c-src tyrosine kinase) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. CSK is a tyrosine kinase known to phosphorylate LCK, FYN and LYN. Varying concentrations of CSK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. |
| Molecular Mass : |
50.5 kDa |
| AA Sequence : |
MSAIQAAWPSGTECIAKYNFHGTAE QDLPFCKGDVLTIVAVTKDPNWYKA KNKVGREGIIPANYVQKREG VKAGTKLSLMPWFHGKITREQAERL LYPPETGLFLVRESTNYPGDYTLCV SCDGKVEHYRIMYHASKLSI DEEVYFENLMQLVEHYTSDADGLCT RLIKPKVMEGTVAAQDEFYRSGWAL NMKELKLLQTIGKGEFGDVM LGDYRGNKVAVKCIKNDATAQAFLA EASVMTQLRHSNLVQLLGVIVEEKG GLYIVTEYMAKGSLVDYLRS RGRSVLGGDCLLKFSLDVCEAMEYL EGNNFVHRDLAARNVLVSEDNVAKV SDFGLTKEASSTQDTGKLPV KWTAPEALREKKFSTKSDVWSFGIL LWEIYSFGRVPYPRIPLKDVVPRVE KGYKMDAPDGCPPAVYEVMK
NCWHLDAAMRPSFLQLREQLEHIKT HELHLTRTRPLEQKLISEEDLAAND ILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, Protein Kinase |
| Protein Pathways : |
Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton |
