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Recombinant Human Midkine, His-tagged

Cat. No.: MDK-384H
Product Overview: Recombinant Human Midkine produced inE. coliis fused with N-terminal His-Tag.
Description: Midkine (MK, MDK), or Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair.
Amino Acid Sequence: Recombinant Human Midkine has a molecular mass of 14.6 kDa protein and contains 121 amino acid residues of the Midkine human and 10 additional amino acid residues –His-Tag. MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Source: E. coli.
Purity: Greater than 95% as determined by SDS-PAGE.
Format: Lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.1M NaCl, pH 7.2.
Specificity: The amino acid sequence of the Midkine human recombinant is 100% homologous to the amino acid sequence of the Midkine human without signal sequence.
Applications: Western blotting, ELISA.
Reconstitution: Add 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely.
Storage: Store lyophilized protein at -20℃. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°Cfor a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name: MDK midkine (neurite growth-promoting factor 2) [ Homo sapiens ]
Synonyms: MDK; midkine (neurite growth-promoting factor 2); MK; NEGF2; FLJ27379; midkine; ARAP; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting protein; neurite outgrowth-promoting factor; MK1; Neurite outgrowth-promoting factor 2; OTTHUMP000001970422; OTTHUMP000001970412; OTTHUMP000001970452
Gene ID: 4192
mRNA Refseq: NM_001012333
Protein Refseq: NP_001012333
MIM: 162096
UniProt ID: P2174
Chromosome Location: 11p11.2
Function: growth factor activity; heparin binding

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