||Recombinant human NEIL2 was expressed in and purified fromE. coli.
||NEIL2 is a DNA glycosylase involved in base excision repair of oxidative DNA damage. It is a member of the Fpg/Nei/NEIL family of bi-functional DNA glycosylases. Following its glycosylase action, NEIL2 catalyzes sequential β-elimination and δ-elimination reactions via its associated AP lyase. This results in a one-nucleotide gap in DNA containing a 3' phosphate group. A phosphatase is needed to remove the 3'phosphate group before DNA repair synthesis can occur.
||Apparent homogeneity by SDS-PAGE and Coomassie blue staining.
||20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 10% glycerol.
||Store at -70°C. Aliquot to avoid repeated freezing and thawing.