Recombinant Human NEIL2 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: NEIL2-351H
• Product Overview: NEIL2 MS Standard C13 and N15-labeled recombinant protein (NP_659480) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 36.8 kDa
• AA Sequence: MPEGPLVRKFHHLVSPFVGQQVVKT GGSSKKLQPASLQSLWLQDTQVHGK KLFLRFDLDEEMGPPGSSPTPEPPQ KEVQKEGAADPKQVGEPSGQKTLDG SSRSAELVPQGEDDSEYLERDAPAG DAGRWLRVSFGLFGSVWVNDFSRAK KANKRGDWRDPSPRLVLHFGGGGFL AFYNCQLSWSSSPVVTPTCDILSEK FHRGQALEALGQAQPVCYTLLDQRY FSGLGNIIKNEALYRAGIHPLSLGS VLSASRREVLVDHVVEFSTAWLQGK FQGRPQHTQVYQKEQCPAGHQVMKE AFGPEDGLQRLTWWCPQCQPQLSEE PEQCQFSTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NEIL2 nei endonuclease VIII-like 2 (E. coli) [ Homo sapiens (human) ]
• Official Symbol: NEIL2
• Synonyms: NEIL2; nei endonuclease VIII-like 2 (E. coli); nei like 2 (E. coli); endonuclease 8-like 2; FLJ31644; MGC2832; MGC4505; NEH2; nei like 2; nei homolog 2; nei-like protein 2; endonuclease VIII-like 2; DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; NEI2
• Gene ID: 252969
• mRNA Refseq: NM_145043
• Protein Refseq: NP_659480
• MIM: 608933
• UniProt ID: Q969S2