Recombinant Human EFNB1 protein, His-tagged
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|Product Overview :||Recombinant Human EFNB1 (Leu28-Gly232) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Ephrin-B1 (EFNB1) is also known as EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2 (LERK-2), which belongs to the ephrin family. EFNB1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNB1 binds to the receptor tyrosine kinases EPHB1 and EPHA1. EFNB1 binds to, and induce the collapse of, commissural axons/growth cones in vitro. EFNB1 may play a role in constraining the orientation of longitudinally projecting axons. EFNB1 interacts with GRIP1 and GRIP2.|
|Predicted N Terminal :||Leu28|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 23.2 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Leu28-Gly232|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of EFNB1-2237H :||
Human Ephrin-B1, His Tag, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||EFNB1|
|Official Symbol :||EFNB1|
|Synonyms :||EFNB1; ephrin-B1; CFNS, craniofrontonasal syndrome (craniofrontonasal dysplasia) , EPLG2; Elk L; LERK2; EFL-3; LERK-2; ELK ligand; ligand of eph-related kinase 2; eph-related receptor tyrosine kinase ligand 2; CFND; CFNS; EFL3; EPLG2; Elk-L; MGC8782|
|Gene ID :||1947|
|mRNA Refseq :||NM_004429|
|Protein Refseq :||NP_004420|
|UniProt ID :||P98172|
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