Recombinant Human EFNB1 protein, His-tagged EFNB1-2237H

Recombinant Human EFNB1 protein, His-tagged

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Recombinant Human EFNB1 protein, His-tagged

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Cat.No. : EFNB1-2237H
Product Overview : Recombinant Human EFNB1 (Leu28-Gly232) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Ephrin-B1 (EFNB1) is also known as EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2 (LERK-2), which belongs to the ephrin family. EFNB1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNB1 binds to the receptor tyrosine kinases EPHB1 and EPHA1. EFNB1 binds to, and induce the collapse of, commissural axons/growth cones in vitro. EFNB1 may play a role in constraining the orientation of longitudinally projecting axons. EFNB1 interacts with GRIP1 and GRIP2.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Leu28
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 23.2 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Leu28-Gly232
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of EFNB1-2237H :

Human Ephrin-B1, His Tag, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : EFNB1
Official Symbol : EFNB1
Synonyms : EFNB1; ephrin-B1; CFNS, craniofrontonasal syndrome (craniofrontonasal dysplasia) , EPLG2; Elk L; LERK2; EFL-3; LERK-2; ELK ligand; ligand of eph-related kinase 2; eph-related receptor tyrosine kinase ligand 2; CFND; CFNS; EFL3; EPLG2; Elk-L; MGC8782
Gene ID : 1947
mRNA Refseq : NM_004429
Protein Refseq : NP_004420
MIM : 300035
UniProt ID : P98172

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