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| Cat. No.: | Camk2a-746R |
| Product Overview: | Recombinant rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) was expressed inS. frugiperdainsect cells. MW = 33 kDa. |
| Description: | CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage. |
| Source: | S. frugiperda. |
| Purity: | ≥90% by SDS-PAGE. |
| Form: | Liquid. In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween 20 Detergent, pH 7.5. |
| Contaminants: | Phosphatases, proteases: none detected. |
| Activity: | ≥250,000 units/ml. |
| Unit Definition: | One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2, KKALRRQETVDAL, per min at 30°C, pH 7.5. |
| Recommended Reaction Conditions: | 1) Dilute CaM kinase II into 20 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT, and 0.1 mM EDTA, pH 7.5.2. 2)Add 2.4 μM calmodulin, 2 mM CaCl2, 100 µM ATP, and [32P]-γATP at 100 µCi/µmole. 3)Incubate at 30°C. |
| Toxicity: | Standard Handling(MSDS available upon request). |
| Storage: | ≤ -70°C. Avoid freeze/thaw. Following initial thaw, aliquot and freeze (-70°C). |
| Gene Name: | Camk2a calcium/calmodulin-dependent protein kinase II alpha [ Rattus norvegicus ] |
| Synonyms: | Camk2a; calcium/calmodulin-dependent protein kinase II alpha; PKCCD; PK2CDD; calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; EC 2.7.11.17 |
| Gene ID: | 25400 |
| mRNA Refseq: | NM_012920 |
| Protein Refseq: | NP_037052 |
| UniProt ID: | P11275 |
| Chromosome Location: | 18q11 |
| Pathway: | Calcium signaling pathway; ErbB signaling pathway; Glioma; GnRH signaling pathway; Long-term potentiation; Melanogenesis; Neurotrophin signaling pathway; Olfactory transduction; Oocyte meiosis; Wnt signaling pathway |
| Function: | ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; kinase activity; nucleotide binding; protein binding; transferase activity |
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