Active Recombinant Rat Camk2a

• Cat.No.: Camk2a-746R
• Product Overview: Recombinant rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) was expressed inS. frugiperdainsect cells. MW = 33 kDa.

Specification

• Cat. No.: Camk2a-746R
• Description: CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.
• Source: S. frugiperda.
• Purity: ≥90% by SDS-PAGE.
• Form: Liquid. In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween 20 Detergent, pH 7.5.
• Contaminants: Phosphatases, proteases: none detected.
• Activity: ≥250,000 units/ml.
• Unit Definition: One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2, KKALRRQETVDAL, per min at 30°C, pH 7.5.
• Recommended Reaction Conditions: 1) Dilute CaM kinase II into 20 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT, and 0.1 mM EDTA, pH 7.5.2. 2)Add 2.4 μM calmodulin, 2 mM CaCl2, 100 µM ATP, and [32P]-γATP at 100 µCi/µmole. 3)Incubate at 30°C.
• Toxicity: Standard Handling(MSDS available upon request).
• Storage: ≤ -70°C. Avoid freeze/thaw. Following initial thaw, aliquot and freeze (-70°C).

Gene Information

• Gene Name: Camk2a calcium/calmodulin-dependent protein kinase II alpha [ Rattus norvegicus ]
• Synonyms: Camk2a; calcium/calmodulin-dependent protein kinase II alpha; PKCCD; PK2CDD; calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; EC 2.7.11.17
• Gene ID: 25400
• mRNA Refseq: NM_012920
• Protein Refseq: NP_037052
• UniProt ID: P11275
• Chromosome Location: 18q11
• Pathway: Calcium signaling pathway; ErbB signaling pathway; Glioma; GnRH signaling pathway; Long-term potentiation; Melanogenesis; Neurotrophin signaling pathway; Olfactory transduction; Oocyte meiosis; Wnt signaling pathway
• Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; kinase activity; nucleotide binding; protein binding; transferase activity