Cat. No. : |
PDGFC-441H |
Product Overview : |
Recombinant Human Platelet Derived Growth Factor C is expressed inE. coli. |
Description : |
The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet α-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signa primarily through the PDGF-Rα receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-Rβ receptor. Recombinant human PDGF-CC is a 25kDa protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains. |
Source : |
E. coli. |
Molecular Weight : |
25 kDa. |
Endotoxin Level : |
< 0.1 ng per µg of rHu PDGF-CC (1EU/µg). |
Purity : |
Greater than 98% (determined by SDS-PAGE and HPLC analysis). |
Biological Activity : |
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50for this effect is 15-20 ng/ml. |
Formulation : |
Lyophilized from a sterile-filtered solution with no additives. |
Reconstitution : |
Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity. |
Storage/Stability : |
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. |