EZH2 (Y641F) Chemiluminescent Assay Kit
Cat.No. : | Kit-1724 |
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Tag : | Non |
Size : | 96 reactions |
Description : | The EZH2 (Y641F) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH2 (Y641F) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Applications : | Great for studying enzyme kinetics and HTS applications. |
Storage : | Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Kit Components : | EZH2 (Y641F)/EED/SUZ12/RbAp48/AEBP2: 5 µg; -70°C20 µM S-adenosylmethionine: 250 µl; -70°C Primary Antibody 24: 25 µl; -70°C Secondary HRP-labeled antibody 2: 10 µl; -70°C 4x HMT assay buffer 2B: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 8-well strip plate module precoated with histone substrate: 1 plate (12 x 8-well strips) ; +4°C |
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SCAMP5-4086R | Recombinant Rhesus monkey SCAMP5 Protein, His-tagged | +Inquiry |
ARHGAP25-6719Z | Recombinant Zebrafish ARHGAP25 | +Inquiry |
GPR89B-5276H | Recombinant Human GPR89B Protein, GST-tagged | +Inquiry |
◆ Native Proteins | ||
PGN-17S | Native S. aureus PGN Protein | +Inquiry |
AMY2A-8353H | Native Human AMY2A | +Inquiry |
TcdA-188C | Active Native Clostridium difficile Toxin A Protein | +Inquiry |
SERPINA1-5358H | Native Human Serpin Peptidase Inhibitor, Clade A (alpha-1 antiproteinase, antitrypsin), Member 1 | +Inquiry |
KNG1-1844H | Native Human Kininogen 1 | +Inquiry |
◆ Cell & Tissue Lysates | ||
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SW1353-179H | SW1353 Whole Cell Lysate | +Inquiry |
RPA2-2242HCL | Recombinant Human RPA2 293 Cell Lysate | +Inquiry |
LADMAC-966M | LADMAC (mouse bone marrow, producing colony stimulating factor-1) whole cell lysate | +Inquiry |
SK-N-SH-01HL | Human SK-N-SH lysate | +Inquiry |
Not For Human Consumption!
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