| Species : |
Human |
| Source : |
NS0 |
| Tag : |
Non |
| Description : |
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype. |
| Predicted N Terminal : |
Ser293-Pro-Lys-His-His-Ser-Gln-Arg-Ala-Arg |
| Form : |
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Bio-activity : |
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.The ED50 for this effect is typically 2.5-15 ng/mL. |
| Molecular Mass : |
Predicted Molecular Mass: 13 kDa (monomer).SDSPAGE: 22-25 kDa, reducing conditions; 37-41 kDa, nonreducing conditions |
| Endotoxin : |
<0.01 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDSPAGE with silver staining, under reducing conditions. |
| Storage : |
Use a manual defrost freezer and avoid repeated freezethaw cycles.12 months, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 50-200 μg/mL in 4 mM HCl. |
