Active GMP Recombinant Human EGFR protein

• Cat.No.: EGFR-078HG
• Product Overview: GMP Recombinant Human EGFR protein(NP_005219)(620 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Source: HEK293
• Species: Human
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: Fixing cetuximab at 5ug/ml (100ul well) can bind to human epidermal growth factor R (His label) with a linear range of 0.5-15 ng/ml.
• Molecular Mass: Recombinant Human EGFR protein contains 620 aa. It has a predicted MW of 70.3 kDa.
• Protein length: DNA sequence encoding Human EGFR(UniProtKB - P00533) was expressed with His tag at the C-terminal.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; ERBB; ERBB1; HER1; PIG61; mENA
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533