||L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
||The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.
||Screen for inhibitors of hIDO1 enzyme in a cellular context. Note: The expression vector is designed for use in transient transfection protocols. It is NOT SUITABLE for transformation and amplification in bacteria.
||Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||hIDO1 Expression Vector (Component A): 100 µl (50 ng DNA/µl); -20°C IDO1 Assay Medium Supplement 1 (Component B): 200 µl; 4°C IDO1 Assay Medium Supplement 2 (Component C): 200 µl; -20°C IDO1 Detection Reagent (Component D): 200 mg; Room temperature