| Description : |
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Bio-activity : |
The specific activity of IDOI was determined by monitoring kynurenine formation from N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with IDO1. The reaction was carried out at 25°C for 15min in the buffer containing 100mM PBS, pH6.5, 40mM ascorbic acid, 450 units catalase, 20µM methylene blue, and 800µM L-tryptophan as the substrate. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich’s reagent (2% p-dimethylaminobenzaldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation. |
| Molecular Mass : |
45.1 kDa |
| AA Sequence : |
MAHAMENSWTISKEYHIDEEVGFAL PNPQENLPDFYNDWMFIAKHLPDLI ESGQLRERVEKLNMLSIDHL TDHKSQRLARLVLGCITMAYVWGKG HGDVRKVLPRNIAVPYCQLSKKLEL PPILVYADCVLANWKKKDPN KPLTYENMDVLFSFRDGDCSKGFFL VSLLVEIAAASAIKVIPTVFKAMQM QERDTLLKALLEIASCLEKA LQVFHQIHDHVNPKAFFSVLRIYLS GWKGNPQLSDGLVYEGFWEDPKEFA GGSAGQSSVFQCFDVLLGIQ QTAGGGHAAQFLQDMRRYMPPAHRN FLCSLESNPSVREFVLSKGDAGLRE AYDACVKALVSLRSYHLQIV
TKYILIPASQQPKENKTSEDPSKLE AKGTGGTDLMNFLKTVRSTTEKSLL KEGTRTRPLEQKLISEEDLAANDIL DYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
Metabolic pathways, Tryptophan metabolism |
