Luciferase Assay Kit

• Cat.No.: Kit-0527
• Product Overview: The Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive-highly sensitive detection of firefly luciferase activity. • Stable-the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient-simple one-step, homogeneous protocol. • High-throughput-one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility-works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation-does not require a luminometer with injectors.

Specification

• Applications: Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. • High-throughput screening using luciferase reporter cell lines
• Storage: At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible.

Reviews

05/18/2022

Worked well, happy customer.

08/30/2021

Great in lab experiments.

09/04/2020

Satisfied with the purchase.

Q&As

How is luciferase utilized in bioluminescence imaging?

It allows for real-time tracking of cellular and molecular processes in living organisms.

What is the primary function of luciferase in organisms that produce it?

Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.

How does luciferase produce light in a biochemical reaction?

It catalyzes the oxidation of luciferin, releasing light.

In which organisms is luciferase commonly found?

Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.

Are there different types of luciferase, and how do they vary?

Yes, there are different types, varying in their substrate specificity and the color of emitted light.

What is the role of ATP in the luciferase reaction?

ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.

What advantages does the use of luciferase offer in scientific research?

Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.