Recombinant Full Length Photuris pensylvanica (Firefly) Luciferase, Thermostable, His tagged

• Cat.No.: Luciferase-014P
• Product Overview: Recombinant thermostable variant of Photuris pensylvanica (Firefly) Luciferase, also known as Luciferin 4-monooxygenase. Full length, encompassing amino acids 1-545(end). This construct contains a C-terminal His-tag (6×His) The recombinant protein was affinity purified.

Specification

• Species: Firefly
• Source: E.coli
• Tag: His
• Protein Length: 1-545 aa
• Description: Produces green light with a wavelength of 562 nm.
• Form: Aqueous buffer solution.
• Molecular Mass: 61 kDa
• Purity: ≥ 90%
• Notes: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: At least 6 months at -80 centigrade.
• Storage Buffer: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 150 mM Imidazole and 20% glycerol.
• Shipping: At -80 centigrade
• Reference: 1. DE Wet, J.R., et al., Molec. Cell. Bio. 1987 Feb; 7(2):725-737. 2. Brasier, AR., et al., Euro. PMC. 1989 Oct; 7(10):1116-1122.

Gene Information

• Official Symbol: Luciferase
• Synonyms: Luciferase; Luciferin 4-monooxygenase; EC:1.13.12.7
• UniProt ID: Q27757