MMP2 Inhibitor Screening Assay Kit

• Cat.No.: Kit-0320C
• Product Overview: The MMP-2 Colorimetric Drug Discovery Kit is a complete assay system designed to screen MMP-2 inhibitors using a thiopeptide as a chromogenic substrate (AcPLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’- dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε= 13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target. An inhibitor, NNGH12, is also included as a prototypic control inhibitor. Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

Specification

• Storage: Store all components except the microplate (room temperature) at -70°C for the highest stability. The MMP-2 enzyme should be handled carefully in order to retain maximal enzymatic activity. It is stable, in diluted or concentrated form, for several hours on ice. As supplied, MMP-2 enzyme is stable for at least 5 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the MMP-2 into separate tubes and store at -70°C. When setting up the assay, do not maintain diluted components at reaction temperature (e.g. 37°C) for an extended period of time prior to running the assay.
• Size: 96 tests

Reviews

06/19/2021

The solubility and ion exchange ability are very good, which is convenient for subsequent experimental treatment and purification.

05/13/2021

Kinetic parameters such as enzymatic reaction rate and binding affinity were tested in the early stage, and it was found that the protein performed well.

03/16/2021

MMP2 products undergo strict quality control to ensure the consistency and stability of each batch.

Q&As

How to regulate the activity of MMP2 in tumor therapy?

The activity of MMP2 is regulated by inhibitors and antibodies against it to inhibit tumor metastasis and invasion.

How is MMP2 related to metastases that promote disease progression?

MMP2 interacts with some transfer factors, such as VEGF and EGF, to promote tumor metastasis and invasion.

What are the application prospects of MMP2 inhibitors?

Inhibitors of MMP2 can be used as potential drug targets for the treatment of tumors, cardiovascular diseases and other related diseases.

Does MMP2 interact with the tumor microenvironment?

MMP2 interacts with tumor cells, immune cells, blood vessels, etc. in the tumor microenvironment to affect tumor progression and treatment effect.

What is the role of MMP2 in embryonic development?

It is involved in important processes such as morphogenesis, organ development and intraembryonic lymphatic vessel formation during embryonic development.

What are the effects of MMP2 mutations in human disease?

Mutations in MMP2 may affect its protein structure and function, leading to the occurrence and progression of related diseases.