MMP2 Inhibitor Screening Assay Kit
Cat.No. : | Kit-0320C |
Product Overview : | The MMP-2 Colorimetric Drug Discovery Kit is a complete assay system designed to screen MMP-2 inhibitors using a thiopeptide as a chromogenic substrate (AcPLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’- dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε= 13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target. An inhibitor, NNGH12, is also included as a prototypic control inhibitor. Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay. |
- Specification
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Storage : | Store all components except the microplate (room temperature) at -70°C for the highest stability. The MMP-2 enzyme should be handled carefully in order to retain maximal enzymatic activity. It is stable, in diluted or concentrated form, for several hours on ice. As supplied, MMP-2 enzyme is stable for at least 5 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the MMP-2 into separate tubes and store at -70°C. When setting up the assay, do not maintain diluted components at reaction temperature (e.g. 37°C) for an extended period of time prior to running the assay. |
Size : | 96 tests |
Products Types
◆ Recombinant Protein | ||
MMP2-562H | Recombinant Human MMP2 Protein, MYC/DDK-tagged | +Inquiry |
MMP2-03H | Active Recombinant Pre-activated Human MMP2 Protein (110-660) | +Inquiry |
MMP2-1419H | Recombinant Human MMP2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Mmp2-10593M | Recombinant Mouse Mmp2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Mmp2-4100M | Recombinant Mouse Mmp2 Protein, Myc/DDK-tagged | +Inquiry |
◆ Native Protein | ||
MMP2-46H | Native Human MMP-2 | +Inquiry |
MMP2-8455M | Native Mouse MMP2 | +Inquiry |
MMP2-47H | Native Human MMP-2/TIMP-2 Complex | +Inquiry |
◆ Lysates | ||
MMP2-2380HCL | Recombinant Human MMP2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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