MMP2 Inhibitor Screening Assay Kit
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| Cat.No. : | Kit-0320C |
| Product Overview : | The MMP-2 Colorimetric Drug Discovery Kit is a complete assay system designed to screen MMP-2 inhibitors using a thiopeptide as a chromogenic substrate (AcPLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’- dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε= 13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target. An inhibitor, NNGH12, is also included as a prototypic control inhibitor. Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay. |
| Storage : | Store all components except the microplate (room temperature) at -70°C for the highest stability. The MMP-2 enzyme should be handled carefully in order to retain maximal enzymatic activity. It is stable, in diluted or concentrated form, for several hours on ice. As supplied, MMP-2 enzyme is stable for at least 5 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the MMP-2 into separate tubes and store at -70°C. When setting up the assay, do not maintain diluted components at reaction temperature (e.g. 37°C) for an extended period of time prior to running the assay. |
| Size : | 96 tests |
◆ Recombinant Protein
| MMP2-8392HA | Recombinant Human MMP2 protein, GST-tagged, APC labeled | +Inquiry |
| MMP2-182H | Recombinant Human MMP2 Protein, His-tagged | +Inquiry |
| MMP2-5425H | Recombinant Human MMP2 Protein, GST-tagged | +Inquiry |
| Mmp2-44M | Recombinant Mouse MMP-2/TIMP-2 Complex | +Inquiry |
| MMP2-562H | Recombinant Human MMP2 Protein, MYC/DDK-tagged | +Inquiry |
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◆ Native Protein
| MMP2-47H | Native Human MMP-2/TIMP-2 Complex | +Inquiry |
| MMP2-46H | Native Human MMP-2 | +Inquiry |
| MMP2-29475TH | Native Human MMP2 | +Inquiry |
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◆ Lysates
| MMP2-2380HCL | Recombinant Human MMP2 cell lysate | +Inquiry |
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MMP1MMP10MMP11MMP12MMP13MMP14MMP15MMP16MMP17MMP19MMP2MMP20MMP24MMP3MMP7MMP8MMP9MMP21MMP23BMMP26MMP28MMP25MMP27Mmp23Mmp1aMmp1b
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