Native Mouse MMP2

• Cat.No.: MMP2-8455M
• Product Overview: LPA was purified from Mouse Fibroblast.

Specification

• Description: The progelatinase A, a member of the matrix metalloproteinase (MMP) family, has been isolated from macrophages and fibroblasts. Gelatinase A hydrolyses several components of the extracellular matrix, e.g. the collagen types IV, V and XI and gelatin. Progelatinase A complexed via their C-terminal domain with TIMP-2 was isolated from culture media of different cell types. This complex shows both properties of its constituents: Like TIMP-2 it inhibits active matrix metalloproteinases and like gelatinase it shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate). However, its proteolytic activity is less than 10% of that of gelatinase A not complexed with TIMP-2. In contrast to the other MMPs the progelatinase A cannot be activated by the serine proteinase trypsin. Until quite recently a potential natural activator that can transform latent progelatinase A into the active form was unknown. It was shown that the catalytic domain of the membrane type 2-matrix metalloproteinase activates progelatinase A as well as the progelatinase A / TIMP-2 complex, by cleaving the 72 kDa progelatinase A to yield 67 kDa gelatinase A, which is than transformed into 62 kDa gelatinase A. The 62 kDa form is about twice as active as the 67 kDa form towards the Dnp-pepitde (Masui et al.). No significant difference in activity was found between free and complexed gelatinase A forms
• Source: Mouse Fibroblast
• Species: Mouse
• Form: In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl2; 1 μM ZnCl2; 0.05% Brij 35; 0,05% NaN3
• Molecular Mass: 40/42 kDa
• Purity: ≥0% by SDS - PAGE
• Storage: Lyophilized hUK is best stored desiccated below 8°C. Reconstituted hUK can be stored at 4°C for 2-7 days. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.
• Shipping: Dry Ice
• Tag: Non

Gene Information

• Gene Name: Mmp2 matrix metallopeptidase 2 [ Mus musculus ]
• Official Symbol: MMP2
• Synonyms: MMP2; matrix metallopeptidase 2; 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2; GelA; Clg4a; MMP-
• Gene ID: 17390
• mRNA Refseq: NM_008610
• Protein Refseq: NP_032636
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; GnRH signaling pathway, organism-specific biosystem; GnRH signaling pathway, conserved biosystem; IL-3 Signaling Pathway, organism-specific biosystem
• Function: fibronectin binding; hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Reviews

06/19/2021

The solubility and ion exchange ability are very good, which is convenient for subsequent experimental treatment and purification.

05/13/2021

Kinetic parameters such as enzymatic reaction rate and binding affinity were tested in the early stage, and it was found that the protein performed well.

03/16/2021

MMP2 products undergo strict quality control to ensure the consistency and stability of each batch.

Q&As

How to regulate the activity of MMP2 in tumor therapy?

The activity of MMP2 is regulated by inhibitors and antibodies against it to inhibit tumor metastasis and invasion.

How is MMP2 related to metastases that promote disease progression?

MMP2 interacts with some transfer factors, such as VEGF and EGF, to promote tumor metastasis and invasion.

What are the application prospects of MMP2 inhibitors?

Inhibitors of MMP2 can be used as potential drug targets for the treatment of tumors, cardiovascular diseases and other related diseases.

Does MMP2 interact with the tumor microenvironment?

MMP2 interacts with tumor cells, immune cells, blood vessels, etc. in the tumor microenvironment to affect tumor progression and treatment effect.

What is the role of MMP2 in embryonic development?

It is involved in important processes such as morphogenesis, organ development and intraembryonic lymphatic vessel formation during embryonic development.

What are the effects of MMP2 mutations in human disease?

Mutations in MMP2 may affect its protein structure and function, leading to the occurrence and progression of related diseases.