||The NSD1 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD1 activity for screening and profiling applications. The key to the NSD1 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||NSD1 human enzyme: 100 µg; -80°C 400 µM S-adenosylmethionine: 500 µl; -80°C Primary antibody 16: 100 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C4x HMT assay buffer 7: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (transparent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well plate precoated with histone substrate : 1; +4°C