|Description:||The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.|
|Applications:||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.|
|Storage:||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.|
|Kit Components:||PARP1: 2 x 5 µg; -80°C 5x histone mixture: 2 x 1 ml; -80°C 10x Assay Mixture Containing Biotinylated Substrate: 2 x 300 µl; -80°C 10x PARP assay buffer: 2 x 1 ml; -20°C Blocking buffer: 50 ml; +4°C Activated DNA (5x): 2 x 500 µl; -80°C Streptavidin-HRP: 2 x 100 µl; +4°C HRP chemiluminescent substrate A (translucent bottle): 2 x 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 2 x 6 ml; +4°C 384-well plate: 1; Room Temp.|
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