PDE3B Assay Kit II
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Cat.No. : | Kit-1837 |
Product Overview : | Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3B, also known as cGMP-inhibited phosphodiesterase, is involved in mediating the antilipolytic and anti glycogenlytic effects of insulin in adipose and liver tissues. PDE3B can hydrolyze both cAMP and cGMP. |
Size : | 384 reactions |
Description : | The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent. The key to the PDE3B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization. |
Applications : | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
Storage : | At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
◆ Recombinant Protein
Pde3b-4744M | Recombinant Mouse Pde3b Protein, Myc/DDK-tagged | +Inquiry |
PDE3B-742H | Recombinant Human PDE3B Protein, MYC/DDK-tagged | +Inquiry |
PDE3B-4329R | Recombinant Rat PDE3B Protein | +Inquiry |
PDE3B-3492H | Recombinant Human PDE3B protein, His-tagged | +Inquiry |
PDE3B-474H | Recombinant Human PDE3B, GST-tagged, Active | +Inquiry |
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◆ Assay kits
Kit-1836 | PDE3B Assay Kit I | +Inquiry |
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Pde10a PDE11A PDE1A PDE1B Pde1c PDE2A PDE3A PDE3B PDE4A PDE4B PDE4C PDE4D PDE5A PDE6B PDE6C PDE6D PDE7A PDE7B PDE8A PDE9A PDE4DIP PDE12 PDE6A PDE8B PDE6G PDE6H
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