||Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3B, also known as cGMP-inhibited phosphodiesterase, is involved in mediating the antilipolytic and anti glycogenlytic effects of insulin in adipose and liver tissues. PDE3B can hydrolyze both cAMP and cGMP.
||The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent. The key to the PDE3B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
||At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||PDE3B recombinant enzyme: 1 µg; -80°C FAM-Cyclic-3′, 5′-AMP (20 µM): 50 µl; -80°C PDE assay buffer: 25 ml; -20°C Binding Agent: 250 µl; +4°C Binding Agent Diluent: 25 ml; +4°C Black, low binding, microtiter plate: 1; Room temp.