Recombinant Active Human IL33 Protein, His-tagged(C-ter)

• Cat.No.: IL33-191H
• Product Overview: Recombinant Active Human IL33 Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
• Source: E. coli
• Species: Human
• Tag: His(C-ter)
• Form: Powder
• Bio-activity: Determined by its ability to binding with recombinant ST2L (IL-33 receptor). The ED50 for this effect is < 54 ng/mL. Determined by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is < 0.1 ng/mL. The specific activity of recombinant human IL-33 is approximately > 4 x 10^7 IU/mg.
• AA Sequence: MSITGISPITEYLASLSTYNDQSIT FALEDESYEIYVEDLKKDEKKDKVL LSYYESQHPSNESGDGVDGKMLMVT LSPTKDFWLHANNKEHSVELHKCEK PLPDQAFFVLHNMHSNCVSFECKTD PGVFIGVKDNHLALIKVDSSENLCT ENILFKLSET
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 7.4)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: IL33 interleukin 33 [ Homo sapiens ]
• Official Symbol: IL33
• Synonyms: IL33; interleukin 33; C9orf26, chromosome 9 open reading frame 26 (NF HEV); interleukin-33; DKFZp586H0523; DVS27; DVS27 related protein; IL1F11; interleukin 1 family; member 11; NF HEV; nuclear factor for high endothelial venules; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor from high endothelial venules; NF-HEV; NFEHEV; C9orf26; RP11-575C20.2
• Gene ID: 90865
• mRNA Refseq: NM_001199640
• Protein Refseq: NP_001186569
• MIM: 608678
• UniProt ID: O95760

Reviews

10/16/2021

Good solubility and is easy to operate and purify.

05/03/2021

Effectively mimics the function of the target protein in vitro.

03/18/2020

IL33 has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.

Q&As

Which immune cells can recombinant IL33 be used to detect?

It can be used to detect immune cells such as macrophages and dendritic cells.

In what research fields can recombinant IL33 be applied?

This protein applied in immunology, drug research, life science and other fields.

What is the action mechanism of IL33?

Regulates the activity of immune cells by binding to receptors to stimulate the production of inflammatory factors such as interferon and TNF-α.

Besides treating asthma and hay fever, what other diseases can be treated with IL33?

IL33 can also be used to treat a variety of inflammatory diseases, such as inflammatory bowel disease, psoriasis and so on.

How to prepare IL33?

The preparation of IL33 requires first cloning the IL33 gene into the expression vector, and then the expression vector is expressed and purified in the cell culture medium.

How long does it take to treat asthma with IL33?

Treating asthma with recombinant IL33 takes several weeks to several months to be effective, depending on the severity of the condition and the individual patient.