Active Recombinant Canine IL5 Protein, His-tagged

• Cat.No.: IL5-2177C
• Product Overview: Recombinant Canine IL5 Protein extracellular domain (NP_001006951.1) (Met 1-Ser 134) was expressed in baculovirus-Insect cells with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect cell
• Species: Canine
• Tag: His
• Form: Lyophilized Powder
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-8 ng/mL.
• Molecular Mass: The recombinant canine IL5 consists of 123 amino acids and has a predicted molecular mass of 14.5 kDa. As a result of glycosylation, the apparent molecular mass of the canine IL5 is approximately 17-19 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage buffer: 20mM Tris, 500mM NaCl, pH 8.5, 10% gly

Gene Information

• Gene Name: IL5 interleukin 5 [ Canis lupus familiaris (dog) ]
• Official Symbol: IL5
• Synonyms: IL-5; T-cell replacing factor; TRF; eosinophil differentiation factor; interleukin 5 (colony-stimulating factor, eosinophil)
• Gene ID: 403790
• mRNA Refseq: NM_001006950.1
• Protein Refseq: NP_001006951.1
• UniProt ID: Q95J76