Species : |
Human |
Source : |
Hordeum Vulgare |
Tag : |
Non |
Description : |
IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors. |
Predicted N Terminal : |
His |
Form : |
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2 |
Bio-activity : |
Bioactivity is assayed by measuring the dose dependent effect of human IL-5 on proliferation of TF-1 cells. The EC50 for this batch of recombinant human IL-5 was determined to be 1.51 ng/ml, corresponding to 0.66x 10E6 U/mg. |
Molecular Mass : |
Recombinant human IL-5 contains 115 amino acids and a 16 a.a. histidine-based tag for a total length of 131 a.a. and has a predicted molecular mass of 15.3 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 22 and 23 kDa in SDS-PAGE. |
Endotoxin : |
Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay. |
Purity : |
>98% by SDS-PAGE gel analysis. |
Storage : |
The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |