Recombinant Cynomolgus FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged

• Cat.No.: FCGRT & B2M-1250C
• Product Overview: Recombinant Cynomolgus FCGRT (Q8SPV9)(Met 1-Ser 297) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Met1-Ser297 & Met1-Met119
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its ability to bind human IgG1 in functional ELISA. 2.Loaded Cynomolgus FcRn recombinant protein on His1K Biosensor, can bind IgG1 Fc with an affinity constant of 0.032 μM as determined in a BLI assay (ForteBio Octet Red384). 3.Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.09 μM as determined in an SPR assay (Biacore T200). 4.Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.18 μM as determined in an SPR assay (Biacore T200).
• Molecular Mass: The recombinant heterodimer of cynomolgus FCGRT&B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.9+ 11.6) KDa. The apparent molecular mass of cynomolgus FCGRT&B2M heterodimer is approximately 35 & 12 KDa respectively in SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.