Recombinant Full Length Human ADAMTS3 Protein, C-Flag-tagged

• Cat.No.: ADAMTS3-1829HFL
• Product Overview: Recombinant Full Length Human ADAMTS3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease, a member of the procollagen aminopropeptidase subfamily of proteins, may play a role in the processing of type II fibrillar collagen in articular cartilage.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 135.4 kDa
• AA Sequence: MVLLSLWLIAAALVEVRTSADGQAG NEEMVQIDLPIKRYREYELVTPVST NLEGRYLSHTLSASHKKRSA RDVSSNPEQLFFNITAFGKDFHLRL KPNTQLVAPGAVVEWHETSLVPGNI TDPINNHQPGSATYRIRKTE PLQTNCAYVGDIVDIPGTSVAISNC DGLAGMIKSDNEEYFIEPLERGKQM EEEKGRIHVVYKRSAVEQAP IDMSKDFHYRESDLEGLDDLGTVYG NIHQQLNETMRRRRHAGENDYNIEV LLGVDDSVVRFHGKEHVQNY LLTLMNIVNEIYHDESLGVHINVVL VRMIMLGYAKSISLIERGNPSRSLE NVCRWASQQQRSDLNHSEHH DHAIFLTRQDFGPAGMQGYAPVTGM CHPVRSCTLNHEDGFSSAFVVAHET GHVLGMEHDGQGNRCGDETA MGSVMAPLVQAAFHRYHWSRCSGQE LKRYIHSYDCLLDDPFDHDWPKLPE LPGINYSMDEQCRFDFGVGY KMCTAFRTFDPCKQLWCSHPDNPYF CKTKKGPPLDGTECAAGKWCYKGHC MWKNANQQKQDGNWGSWTKF GSCSRTCGTGVRFRTRQCNNPMPIN GGQDCPGVNFEYQLCNTEECQKHFE DFRAQQCQQRNSHFEYQNTK HHWLPYEHPDPKKRCHLYCQSKETG DVAYMKQLVHDGTHCSYKDPYSICV RGECVKVGCDKEIGSNKVED KCGVCGGDNSHCRTVKGTFTRTPRK LGYLKMFDIPPGARHVLIQEDEASP HILAIKNQATGHYILNGKGE EAKSRTFIDLGVEWDYNIEDDIESL HTDGPLHDPVIVLIIPQENDTRSSL TYKYIIHEDSVPTINSNNVI QEELDTFEWALKSWSQCSKPCGGGF QYTKYGCRRKSDNKMVHRSFCEANK KPKPIRRMCNIQECTHPLWV AEEWEHCTKTCGSSGYQLRTVRCLQ PLLDGTNRSVHSKYCMGDRPESRRP CNRVPCPAQWKTGPWSECSV TCGEGTEVRQVLCRAGDHCDGEKPE SVRACQLPPCNDEPCLGDKSIFCQM EVLARYCSIPGYNKLCCESC SKRSSTLPPPYLLEAAETHDDVISN PSDLPRSLVMPTSLVPYHSETPAKK MSLSSISSVGGPNAYAAFRP NSKPDGANLRQRSAQQAGSKTVRLV TVPSSPPTKRVHLSSASQMAAASFF AASDSIGASSQARTSKKDGK IIDNRRPTRSSTLER TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Secreted Protein

Gene Information

• Gene Name: ADAMTS3 ADAM metallopeptidase with thrombospondin type 1 motif 3 [ Homo sapiens (human) ]
• Official Symbol: ADAMTS3
• Synonyms: HKLLS3; ADAMTS-4
• Gene ID: 9508
• mRNA Refseq: NM_014243.3
• Protein Refseq: NP_055058.2
• MIM: 605011
• UniProt ID: O15072