Recombinant Full Length Human APEX1 Protein, C-Flag-tagged

• Cat.No.: APEX1-2021HFL
• Product Overview: Recombinant Full Length Human APEX1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.4 kDa
• AA Sequence: MPKRGKKGAVAEDGDELRTEPEAKK SKTAAKKNDKEAAGEGPALYEDPPD QKTSPSGKPATLKICSWNVD GLRAWIKKKGLDWVKEEAPDILCLQ ETKCSENKLPAELQELPGLSHQYWS APSDKEGYSGVGLLSRQCPL KVSYGIGDEEHDQEGRVIVAEFDSF VLVTAYVPNAGRGLVRLEYRQRWDE AFRKFLKGLASRKPLVLCGD LNVAHEEIDLRNPKGNKKNAGFTPQ ERQGFGELLQAVPLADSFRHLYPNT PYAYTFWTYMMNARSKNVGW RLDYFLLSHSLLPALCDSKIRSKAL GSDHCPITLYLAL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair

Gene Information

• Gene Name: APEX1 apurinic/apyrimidinic endodeoxyribonuclease 1 [ Homo sapiens (human) ]
• Official Symbol: APEX1
• Synonyms: APE; APX; APE1; APEN; APEX; HAP1; REF1
• Gene ID: 328
• mRNA Refseq: NM_080648.3
• Protein Refseq: NP_542379.1
• MIM: 107748
• UniProt ID: P27695