Recombinant Full Length Human APEX2 Protein, C-Flag-tagged

• Cat.No.: APEX2-1035HFL
• Product Overview: Recombinant Full Length Human APEX2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.2 kDa
• AA Sequence: MLRVVSWNINGIRRPLQGVANQEPS NCAAVAVGRILDELDADIVCLQETK VTRDALTEPLAIVEGYNSYF SFSRNRSGYSGVATFCKDNATPVAA EEGLSGLFATQNGDVGCYGNMDEFT QEELRALDSEGRALLTQHKI RTWEGKEKTLTLINVYCPHADPGRP ERLVFKMRFYRLLQIRAEALLAAGS HVIILGDLNTAHRPIDHWDA VNLECFEEDPGRKWMDSLLSNLGCQ SASHVGPFIDSYRCFQPKQEGAFTC WSAVTGARHLNYGSRLDYVL GDRTLVIDTFQASFLLPEVMGSDHC PVGAVLSVSSVPAKQCPPLCTRFLP EFAGTQLKILRFLVPLEQSP VLEQSTLQHNNQTRVQTCQNKAQVR STRPQPSQVGSSRGQKNLKSYFQPS PSCPQASPDIELPSLPLMSA LMTPKTPEEKAVAKVVKGQAKTSEA KDEKELRTSFWKSVLAGPLRTPLCG GHREPCVMRTVKKPGPNLGR
  RFYMCARPRGPPTDPSSRCNFFLWS RPSTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair

Gene Information

• Gene Name: APEX2 apurinic/apyrimidinic endodeoxyribonuclease 2 [ Homo sapiens (human) ]
• Official Symbol: APEX2
• Synonyms: APE2; XTH2; ZGRF2; APEXL2
• Gene ID: 27301
• mRNA Refseq: NM_014481.4
• Protein Refseq: NP_055296.2
• MIM: 300773
• UniProt ID: Q9UBZ4