Recombinant Full Length Human CHMP4B Protein, C-Flag-tagged

• Cat.No.: CHMP4B-707HFL
• Product Overview: Recombinant Full Length Human CHMP4B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 24.8 kDa
• AA Sequence: MSVFGKLFGAGGGKAGKGGPTPQEA IQRLRDTEEMLSKKQEFLEKKIEQE LTAAKKHGTKNKRAALQALK RKKRYEKQLAQIDGTLSTIEFQREA LENANTNTEVLKNMGYAAKAMKAAH DNMDIDKVDELMQDIADQQE LAEEISTAISKPVGFGEEFDEDELM AELEELEQEELDKNLLEISGPETVP LPNVPSIALPSKPAKKKEEE
  DDDMKELENWAGSMTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Endocytosis

Gene Information

• Gene Name: CHMP4B charged multivesicular body protein 4B [ Homo sapiens (human) ]
• Official Symbol: CHMP4B
• Synonyms: SNF7; CTPP3; Shax1; CHMP4A; SNF7-2; VPS32B; CTRCT31; Vps32-2; C20orf178; dJ553F4.4
• Gene ID: 128866
• mRNA Refseq: NM_176812.5
• Protein Refseq: NP_789782.1
• MIM: 610897
• UniProt ID: Q9H444