| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Flag |
| Protein Length : |
2-422 aa(end) |
| Description : |
The Cereblon (CRBN) protein via its interaction with the DNA damage-binding protein-1 (DDB1), Cullin 4 (Cul4A or Cul4B), and regulator of Cullins 1 (RoC1) forms a functional E3 Ub ligase complex. In this complex, Cereblon functions as a substrate receptor that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Like most E3 ligases, Cereblon complex ubiquitinates itself and its auto-ubiquitination promotes its ligase activity. Cereblon complex is involved in many biological processes including cell proliferation and apoptosis, and it has been extensively used in targeted protein degradation (PROTAC®), where CRBN binds to the protein of interest and leads to its degradation. This approach has shown promising results in the treatment of cancer. |
| Form : |
Aqueous buffer solution. 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 100 μg ml FLAG peptide. |
| Molecular Mass : |
51 kDa |
| Purity : |
≥70% |
| Applications : |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Storage : |
-80 centigrade |
| Concentration : |
0.31 mg/ml |
| Use/Stability : |
At least 6 months at -80 centigrade. Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles. |
